Analysis of Genetic Polymorphism Association of Interleukin-8 (+781 C/T) in Breast Cancer Patients

Abstract

Globally, Breast cancer is the major mortality risk among the females. Its complex etiology includes a variety of risk factors, including genetic predisposition. Interleukin-8 (IL-8), a chemoattractant, has received attention for its possible prognostic and histopathological importance in breast cancer. The aim of this research was to look at the relationship between environmental variables, genetic variation (IL-8, +781 C/T) polymorphism, and breast cancer in Pakistan. The study had two goals: first, to examine the impact of environmental variables on breast cancer risk, and second, to investigate the IL-8 (+781 C/T) polymorphism and breast cancer relationship susceptibility. Samples of blood in this study group were taken from 100 breast cancer patients and 50 healthy control females across several institutions using a thorough case-control methodology. Specific primers were used to amplify the SNP through PCR; and agarose gel electrophoresis were used to genotype the IL-8 (+781 C/T) polymorphism. In breast cancer patients, the genotype distribution revealed that 67% had the CT genotype, and 33% had the TT genotype. The T allele was more common in both groups, accounting for 66.5%. The findings revealed a favorable relationship between age and CRP (r = 0.387***) as well as CTC (r = 0.369***). Notably, CRP correlated positively with CTC (r = 0.270***), indicating a reciprocal impact between both variables. According to the findings of this extensive study, the CT genotype was substantially more prevalent in breast cancer patients with the IL-8 +781 C/T polymorphism. Individuals with the TT genotype of IL-8 +781 C/T were shown to be more sensitive to breast cancer.